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目的就强酸性电解质水对粪肠球菌生物膜杀菌作用进行体外研究。方法粪肠球菌生物膜在体外培养24h,然后等量分为三组,分别采用去离子水、20mL/L氯已定、强酸性电解质水来进行处理,在处理后的3个时间点进行荧光染色处理,对各组生物膜中死菌、活菌的变化用激光共聚焦显微镜来予以观察。结果粪肠球菌生物膜在强酸性电解质水处理后5min,以死菌为主,叠加同一视野后呈橘红色;处理10min,15min后,基本均为死菌,叠加同一视野后呈红色。与去离子水处理以及20mL/L氯己定处理相比,强酸性电解质水处理各时间点的活菌数都要明显更低一些,存在着较为明显的差异,差异具有统计学意义(P<0.05)。结论强酸性电解质水对粪肠球菌生物膜的杀菌作用较强,远远强于去离子水和20mL/L氯己定,值得推广。
Objective To study the bactericidal effect of strong acid electrolyte water on Enterococcus faecalis biofilm in vitro. Methods The Enterococcus faecalis biofilms were cultured in vitro for 24 h and then divided into three equal groups. The cells were treated with deionized water, 20 mL / L chlorhexidine, and strongly acidic electrolyzed water respectively. Fluorescence was performed at three time points Dyeing treatment, the death of each group of biofilms, the changes of viable cells were observed with a confocal laser microscope. Results The Enterococcus faecalis biofilms were mainly dead with 5 min after the treatment of strong acid electrolytes, and they were orange-red after being superposed on the same field of view. After treated for 10 min and 15 min, they were all dead, and were red after superimposing the same field of view. Compared with deionized water treatment and 20mL / L chlorhexidine treatment, the number of viable cells in the treatment of strong acid electrolytes was significantly lower at different time points, with significant differences (P < 0.05). Conclusion Strong acid electrolyzed water has a strong bactericidal effect on Enterococcus faecalis biofilm, which is much stronger than that of deionized water and 20 mL / L chlorhexidine, which deserves promotion.