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目的探讨生物转化酶类细胞色素P450(CYP)基因CYP1A1m1、m2和谷胱甘肽转硫酶(GST)M1基因多态性与前列腺癌易感性的关系。方法采用寡核苷酸芯片对83例前列腺癌患者(前列腺癌组)和115例非前列腺癌对照者(对照组)的中国汉族人基因组DNA进行CYP1A1、GSTM1基因多态性分析。结果GSTM1缺失型在前列腺癌组和对照组均为48例(57.8%,41.7%),两组比较差异有统计学意义(χ2=4.99,P=0.025),GSTM1缺失型使患前列腺癌的危险度增加1.9倍(95%CI=1.10~3.40)。GSTM1缺失型随着前列腺癌的分期、分级的提高,其相对危险度明显提高。前列腺癌组CYP1A1基因的两个多态位点m1、m2基因型频率和等位基因的频率与对照组相比均无统计学意义(P>0.05)。结论中国汉族人群GSTM1缺失型可能与前列腺癌的发病风险相关,与前列腺癌的分级、分期有关,对临床预测前列腺癌预后可能有一定意义。
Objective To investigate the relationship between CYP1A1m1, m2 and glutathione S-transferase (M1) gene polymorphisms of biotransforming enzymes CYP and susceptibility to prostate cancer. Methods The genomic DNA of CYP1A1 and GSTM1 in 83 Chinese Han patients with prostate cancer (prostate cancer group) and 115 non-prostate cancer controls (control group) were analyzed by oligonucleotide microarray. Results There were 48 cases (57.8%, 41.7%) with GSTM1 deletion in prostate cancer group and control group. There was significant difference between the two groups (χ2 = 4.99, P = 0.025). GSTM1 deletion caused the risk of prostate cancer 1.9 times (95% CI = 1.10 ~ 3.40). With the staging and grading of prostate cancer, the relative risk of GSTM1 deletion increased significantly. There were no significant differences in the frequencies of m1 and m2 genotypes and alleles between CYP1A1 gene and prostate cancer in the prostate cancer group (P> 0.05). Conclusions The deletion of GSTM1 in Chinese Han population may be related to the risk of prostate cancer, which is related to the grade and stage of prostate cancer. It may be of clinical significance to predict the prognosis of prostate cancer.