对复合氨基酸注射液 (17AA)中含量较低的 N-乙酰 - L-酪氨酸及 N-乙酰 - L-半胱氨酸用 HPL C法进行测定。采用阳离子交换色谱柱 ,以水 (取 0 .0 5 mol/ L 硫酸 1ml置 10 0 0 ml量瓶中以水定容 ,调节 p H至 3.0 )为流动相 ,检测波长为 2 10 nm。该方法在 10～ 10 0 μg/ m l范围呈良好的线性 ,相关系数分别为 0 .9996和 0 .9999,平均回收率分别为 99.94%和 99.72 % ,RSD分别为 0 .42 %及 1.19%。 The content of N-acetyl-L-tyrosine and N-acetyl-L-cysteine ​​in compound amino acid injection (17AA) was determined by HPL-C method. Cation exchange column was used. The mobile phase consisted of water (take 1 ml of 0.05 mol / L sulfuric acid in 100 ml volumetric flask and adjust p H to 3.0) with a detection wavelength of 210 nm. The method showed a good linearity in the range of 10 ~ 100 μg / ml with correlation coefficients of 0.9996 and 0.9999, respectively. The average recoveries were 99.94% and 99.72%, respectively, with RSDs of 0.42% and 1.19%, respectively.