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目的 :研究凋亡细胞在脑缺血及再灌注后的时空表达方式及原癌基因 c-fos的表达 ,以期探讨脑缺血时 c-fos表达与细胞凋亡的关系。方法 :用线栓法建立局灶性脑缺血再灌注模型 ,DNA缺口末端标记法原位检测细胞凋亡 ,免疫组化检测 c-fos的表达。结果 :缺血侧鼠脑凋亡细胞数随再灌注时间延长而增多 ,凋亡细胞主要分布于额顶叶皮层与纹状体 ,以梗死灶边缘区密度最高。正常组、假手术组未见 c-fos表达 ,缺血及再灌注各组缺血对侧有少量 c-fos表达 ,缺血侧 c-fos阳性细胞数随再灌注时间延长而增多。 c-fos阳性细胞主要分布于缺血侧新皮层与纹状体 ,扣带回皮层、部分丘脑与海马也见较多表达。结论 :脑内缺血性神经元凋亡可能与 c-fos表达水平改变有关
OBJECTIVE: To investigate the temporal and spatial expression of the apoptotic cells and the expression of proto-oncogene c-fos after cerebral ischemia and reperfusion in order to explore the relationship between the expression of c-fos and apoptosis in cerebral ischemia. Methods: Focal cerebral ischemia / reperfusion model was established by thread occlusion. Apoptosis was detected by DNA nick end labeling in situ. The expression of c-fos was detected by immunohistochemistry. Results: The number of apoptotic cells in the ischemic rat increased with the prolongation of reperfusion time. The apoptotic cells mainly distributed in the frontal cortex and striatum, with the highest density in the marginal zone of infarction. No c-fos expression was observed in the normal and sham-operated groups. A small amount of c-fos was expressed on the contralateral side of the ischemic and reperfused groups, while the number of c-fos positive cells on the ischemic side increased with the reperfusion time. c-fos positive cells mainly distributed in the ischemic neocortex and striatum, cingulate cortex, some of the thalamus and hippocampus also see more expression. Conclusion: The apoptosis of cerebral ischemic neurons may be related to the change of c-fos expression