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目的:探讨持续递增负荷跑台运动和游泳力竭法复制运动三联症(FAT)大鼠模型,及加用放血+环磷酰胺腹腔注射法复制气血亏虚证FAT大鼠模型饮食紊乱与物质代谢紊乱的调控机制。方法:SD大鼠随机分为空白组(正常饮食)、模型1组(持续递增负荷跑台运动+限制饮食)、模型2组(持续递增负荷跑台运动+限制饮食+放血+环磷酰胺)、模型3组(持续递增力竭游泳+限制饮食)、模型4组(持续递增力竭游泳+限制饮食+放血+环磷酰胺)5组,检测造模前后大鼠体质量及8周后大鼠血浆三碘甲状腺原氨酸(T3)、甲状腺素(T4)、促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)和下丘脑食欲素A含量。结果:与空白组比较,模型1~4组大鼠T3含量和CRH含量变化不明显,差异无统计学意义(P>0.05);模型1~4组大鼠造模后体质量较低,T4含量显著性减少,食欲素A含量显著性降低,ACTH含量显著性增加,差异均有统计学意义(P<0.05,P<0.01)。与模型1组比较,模型2组大鼠造模后体质量、ACTH含量、T4含量、CORT含量均较低,差异均有统计学意义(P<0.05,P<0.01);模型3组大鼠T4含量和CORT含量均较低,差异均有统计学意义(P<0.01,P<0.05)。与模型3组比较,模型4组大鼠造模后体质量、T4含量、ACTH含量、CORT含量均较低,差异均有统计学意义(P<0.01)。结论:持续递增负荷跑台运动和游泳力竭法均可复制FAT大鼠模型,大鼠体质量增加缓慢、下丘脑-垂体-甲状腺(HPT)轴功能降低和下丘脑-垂体-肾上腺皮质(HPA)轴功能亢进;与递增负荷训练模型FAT大鼠比较,气血亏虚证FAT模型大鼠体质量增加更小、HPT轴功能更低而HPA功能亢进不明显。
OBJECTIVE: To investigate the effects of continuous increasing load treadmill exercise and swimming exhaustive exercise triad (FAT) rat model and dietary disorder and substance of fat rat model of Qi deficiency and blood stasis syndrome with blood transfusion and cyclophosphamide intraperitoneal injection Regulatory mechanism of metabolic disorders. Methods: Sprague-Dawley rats were randomly divided into blank group (normal diet), model group 1 (continuous increasing treadmill exercise + limited diet), model group 2 (continuous increasing treadmill exercise + limiting diet + bleeding + cyclophosphamide) , Model group 3 (continuous increasing exhaustive swimming + limiting diet), model 4 (continuous increasing exhaustive swimming + limiting diet + bleeding + cyclophosphamide) 5 groups, before and after the establishment of rat body mass and 8 weeks after the big (T3), thyroxine (T4), corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT) and hypothalamic orexin- Results: Compared with the blank group, the contents of T3 and CRH did not change significantly in model group 1 to 4 (P> 0.05), while the body weight of model 1 to 4 group was lower after T4 Significant reduction in content, significant reduction of orexin A content, ACTH content was significantly increased, the difference was statistically significant (P <0.05, P <0.01). Compared with model group 1, the body weight, ACTH content, T4 content and CORT content in model group 2 were lower than those in model group 1 (P <0.05, P <0.01) T4 content and CORT content were lower, the differences were statistically significant (P <0.01, P <0.05). Compared with the model group 3, the body weight, T4 content, ACTH content and CORT content in the model group 4 rats were lower after modeling (P <0.01). CONCLUSION: FAT rat model can be replicated by both continuous increasing treadmill exercise and exhaustive swimming. Rat body weight increases slowly, hypofunction of hypothalamus-pituitary-thyroid (HPT) axis and hypothalamic-pituitary-adrenal cortex (HPA ) Axis function hyperthyroidism; Compared with the fat load training model FAT rats, FAT model rats with qi deficiency blood deficiency increased less in body weight, HPT axis function lower and HPA hyperthyroidism is not obvious.