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目的 :探讨化疗前后卵巢癌细胞及外周血淋巴细胞凋亡率及凋亡因子Fas和Apo2 7的含量及其临床意义。方法 :从 75例化疗前后卵巢癌患者的腹水中提取富含癌细胞的悬液 ,用AnnexinV -FITC/PI双染法分辨凋亡细胞及坏死细胞 ,并以两种凋亡因子单克隆抗体Fas和Apo2 7标记上述细胞 ,均用流式细胞仪检测。结果 :化疗后卵巢癌细胞凋亡率显著高于化疗前的临床疗效好 ,反之疗效差 ;被Fas及Apo2 7标记的癌细胞率显著增加者临床疗效好 ,反之疗效差 ;化疗后外周血淋巴细胞凋亡率及凋亡因子有不同程度的增加。结论 :通过对化疗前后卵巢癌细胞凋亡程度的检测 ,有助于判断化疗效果 ,有重要的临床意义。
Objective: To investigate the apoptosis rate of ovarian cancer cells and peripheral blood lymphocytes and the content of apoptosis factors Fas and Apo2 7 before and after chemotherapy and their clinical significance. Methods: Apoptosis-rich cells and necrotic cells were isolated from 75 patients with ascites from patients with ovarian cancer before and after chemotherapy. Apoptotic cells and necrotic cells were identified by Annexin V-FITC / PI double staining. And Apo2 7 labeled cells were detected by flow cytometry. Results: The apoptosis rate of ovarian cancer cells after chemotherapy was significantly higher than that before chemotherapy, while the therapeutic effect was poor. On the contrary, the curative effect of cancer cells marked by Fas and Apo2 7 was good, while the curative effect was poor. On the other hand, Apoptosis rate and apoptosis factor have different degrees of increase. Conclusion: The detection of ovarian cancer cell apoptosis before and after chemotherapy is helpful to judge the effect of chemotherapy, which has important clinical significance.