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目的对患者白细胞介素-6(IL-6)、基因启动子-572C/G、基因启动子-634C/G在血清中的表达与脑梗死病情之间的关系针对地区性进行探讨。方法采用PCR-RFLP分析法对患者IL-6及对照组健康人群基因启动因子-572C/G、-634C/G检测,酶联免疫法检测IL-6血清表达水平。结果脑梗死患者血清中IL-6水平明显高于对照组,IL-6基因启动因子基因型频率与等位基因频率与对照组相比具有显著差异,通过基因频率风险分析得出脑梗塞G等位基因与C等位基因携带者风险相差1.6215倍,携带G等位基因患者血清中IL-6表达水平明显高于非携带者。结论基因启动子-572G/C与脑梗死发病具有相关性且可能为脑梗死发病的遗传易感基因,G等位基因个体通过促进IL-6表达增高而提高脑梗死患者各项风险。
Objective To investigate the relationship between the expression of interleukin-6 (IL-6), gene promoter-572C / G, gene promoter-634C / G in serum and the severity of cerebral infarction. Methods The gene promoter-572C / G and -634C / G of IL-6 and control subjects were detected by PCR-RFLP. The serum IL-6 levels were detected by enzyme-linked immunosorbent assay. Results The level of IL-6 in the serum of patients with cerebral infarction was significantly higher than that of the control group. There was a significant difference in the genotype frequency and allele frequency of IL-6 promoter compared with that of the control group. The risk of cerebral infarction was estimated by genetic frequency risk analysis There was a 1.6215-fold difference between the risk of carriers of C allele and that of carriers of C allele. The levels of IL-6 in sera of patients with G allele were significantly higher than those without carriers. Conclusions The -572G / C gene promoter is associated with the incidence of cerebral infarction and may be a genetic predisposition to cerebral infarction. Individuals with G allele may increase the risk of cerebral infarction by increasing the expression of IL-6.