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果聚糖作为一种重要的渗透调节物质,与植物碳素分配和抗逆性的密切相关。为明确果聚糖在植物生长发育过程中的作用,本研究以菊芋为材料,克隆出果聚糖:果聚糖1-果糖基转移酶1-FFT基因,其CDS长2 025 bp,编码641个氨基酸,该蛋白的分子量(Mw)为73.906 k D,蛋白的等电点(p I)为10.01。进化树分析显示菊芋中1-FFT基因与维氏菊的1-FFT基因进化距离最近,具有较高的保守性。荧光定量检测结果显示,1-FFT基因在菊芋的叶、根、花、茎和块茎中均有表达,其中在块茎中表达量最高。本研究为解析果聚糖合成酶的分子调控机理及功能鉴定提供依据。
As an important osmotic regulator, fructan is closely related to plant carbon distribution and stress resistance. In order to clarify the role of fructans in plant growth and development, this study cloned fructan: fructan 1-fructosyltransferase 1-FFT gene, its CDS length 2025 bp, encoding 641 Amino acids, the molecular weight (Mw) of the protein was 73.906 kD and the isoelectric point (pI) of the protein was 10.01. Phylogenetic tree analysis showed that the 1-FFT gene in Jerusalem artichoke had the closest evolutionary distance to the 1-FFT gene of Vitex japonica, which was highly conserved. Fluorescence quantitative analysis showed that the 1-FFT gene was expressed in leaves, roots, flowers, stems and tubers of Jerusalem artichoke, of which the highest expression was in tuber. This study provides the basis for the analysis of the molecular regulation mechanism and function identification of levansynthase.