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补体系统活化后C5-C9形成末端补体复合物(TCC),其中结合在细胞膜上的称为膜攻击复合物(MAC);存在于血浆中的为Sc5b-9。研究发现,在一些疾病患者的体液中,Sc5b-9含量异常增加。本实验对急性心肌梗塞(AMI)等几种疾病患者血浆中Sc5b-9含量进行检测,并就检测意义作初步探讨。 所有患者均为1991～1992年北京协和医院、朝阳医院的门诊及住院患者,并已确诊。采用EL ISA双夹心法。所用Sc5b-9抗原、兔抗人MAC新抗原抗体及HRP标记抗体均由本室制备。检测51例健康人血浆,其中49例Sc5b-9浓度低于450ng/ml,故采用450ng/ml作为血浆Sc5b-9浓度的正常临界
C5-C9 forms a terminal complement complex (TCC) upon activation of the complement system, where a membrane-bound complex (MAC) that binds to the cell membrane; and Sc5b-9 that is present in the plasma. The study found that in some patients with body fluids, Sc5b-9 levels were abnormally increased. In this experiment, the content of Sc5b-9 in plasma of several patients with acute myocardial infarction (AMI) and other diseases were detected, and the significance of detection was discussed. All patients were from 1991 to 1992 Beijing Union Medical College Hospital, Chaoyang Hospital, outpatient and inpatient, and has been diagnosed. EL ISA double sandwich method. Sc5b-9 antigen used, rabbit anti-human MAC antigen antibody and HRP-labeled antibodies were prepared by this room. Fifty-one healthy subjects were tested for plasma levels of Sc5b-9, which was lower than 450ng / ml, so 450ng / ml was used as the normal threshold for plasma Sc5b-9 concentration