论文部分内容阅读
背景:嗅鞘细胞被证明有修复损伤脊髓的作用,但对其移植后的生物学特性了解不多。目的:观察移植的嗅鞘细胞在损伤脊髓中的迁移方式。设计:随机对照实验。单位:广东省人民医院骨科,中山大学北校区动物实验中心。材料:2个月雄性SD大鼠38只,体质量(350±20)g。方法:实验于2004-02/05在中山大学北校区动物实验中心进行。①取SD大鼠2只用于嗅鞘细胞提取,将嗅鞘细胞用双苯亚甲胺染色。②取SD大鼠36只,随机分为3组,近端组,远端组和对照组,每组12只。近端、远端组建立大鼠胸髓损伤模型。分别于脊髓损伤近端、远端注入嗅鞘细胞悬液;对照组不损伤胸髓,仅注射嗅鞘细胞悬液。主要观察指标:术后1,2,4,6周荧光显微镜下观察脊髓中嗅鞘细胞的分布。结果:36只大鼠,分为3组,术后近端组死亡1只;远端、对照组各死亡2只。进入结果分析29只。各组大鼠脊髓中嗅鞘细胞分布:术后2,4,6周,近端组和远端组的嗅鞘细胞进一步沿着脊髓长轴纵向迁移,最远达8mm,能够穿过瘢痕,到达断端对侧的细胞数量很少;对照组嗅鞘细胞只是局部的扩散,没有迁移。结论:嗅鞘细胞的迁移主要沿着轴突进行,向头端和尾端迁移的数量和速度都没有明显区别,嗅鞘细胞只在损伤的脊髓中进行迁移。
BACKGROUND: Olfactory ensheathing cells have been shown to repair spinal cord injury, but little is known about their biological characteristics after transplantation. Objective: To observe the migration of transplanted olfactory ensheathing cells in injured spinal cord. Design: Randomized controlled experiment. SETTING: Department of Orthopedics, Guangdong Provincial People’s Hospital, North Campus Animal Experiment Center, Sun Yat-sen University. MATERIALS: Thirty-eight male Sprague-Dawley rats of 2 months were enrolled in the study. Body mass (350 ± 20) g. Methods: The experiment was conducted at Animal Experimental Center of North Campus of Sun Yat-sen University from February to May 2004. ① 2 SD rats were taken for olfactory ensheathing cells, olfactory ensheathing cells were exposed with diphenylamine. Thirty-six SD rats were randomly divided into 3 groups: proximal group, distal group and control group, with 12 rats in each group. The proximal and distal groups were established rat model of thoracic injury. Olfactory ensheathing cell suspensions were injected proximally and distally into spinal cord injury respectively. In the control group, the thoracic marrow was not injured and only the olfactory ensheathing cell suspension was injected. MAIN OUTCOME MEASURES: The distributions of olfactory ensheathing cells in the spinal cord were observed under fluorescence microscope 1, 2, 4, and 6 weeks after operation. Results: Thirty-six rats were divided into three groups, one died in the proximal group and two died in the control group. Into the results of analysis of 29. Olfactory ensheathing cells were distributed in the spinal cord of rats in each group. Olfactory ensheathing cells in the proximal and distal groups migrated longitudinally along the long axis of the spinal cord at 2, 4, and 6 weeks after operation, reaching a maximum of 8 mm. They could cross the scar, The number of cells reaching the opposite side of the stump was very small. Olfactory ensheathing cells of the control group were only partially diffused and did not migrate. CONCLUSION: Olfactory ensheathing cells migrate mainly along the axon. There is no significant difference in the number and speed of migration between the tip and the tail. Olfactory ensheathing cells migrate only in injured spinal cord.