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目的:探讨Bcl-2、P53和Caspase-3在不同宫颈病变发生、发展中的作用及其相互关系。方法:对37例宫颈炎、39例宫颈内瘤变、24例宫颈癌患者抽取血液,采用Elisa方法检测血清中Bcl-2、P53和Caspase-3蛋白的含量,采用流式细胞术检测血细胞中Bcl-2、P53和Caspase-3的阳性率,结合临床病理特点分析。结果:宫颈炎患者血清中Bcl-2、P53蛋白含量及血细胞中Bcl-2、P53阳性率明显低于宫颈内瘤变和宫颈癌患者(P<0.01),且宫颈内瘤变患者明显低于宫颈癌患者(P<0.05);宫颈炎患者血清中Caspase-3蛋白含量及血细胞中Caspase-3的阳性率明显高于宫颈内瘤变和宫颈癌患者(P<0.01),且宫颈内瘤变患者明显高于宫颈癌患者(P<0.05)。结论:Bcl-2、P53和Caspase-3蛋白在宫颈癌发生、发展过程中发挥着即独立又协同的作用。
Objective: To investigate the roles and relationships of Bcl-2, P53 and Caspase-3 in the occurrence and development of different cervical lesions. Methods: Blood was collected from 37 cases of cervicitis, 39 cases of cervical neoplasia and 24 cases of cervical cancer. The contents of Bcl-2, P53 and Caspase-3 in serum were detected by Elisa method. The positive rates of Bcl-2, P53 and Caspase-3 were analyzed in combination with clinicopathological features. Results: The levels of Bcl-2 and P53 in sera of patients with cervicitis and the positive rate of Bcl-2 and P53 in blood cells were significantly lower than those in cervical cancer and cervical cancer (P <0.01), and the patients with cervical intraepithelial neoplasia were significantly lower than Cervical cancer patients with Caspase-3 protein content and the positive rate of Caspase-3 in blood cells were significantly higher than those in patients with cervical neoplasia and cervical cancer (P <0.01), and cervical neoplasia Patients were significantly higher than cervical cancer patients (P <0.05). Conclusion: Bcl-2, P53 and Caspase-3 proteins play an independent and synergistic role in the development and progression of cervical cancer.