本研究以与p16失活关系密切的肾癌小鼠为模型,将携带p16基因的腺病毒体外转染小鼠肾癌Renca细胞,观察p16基因修饰后Renca细胞生物学特性的变化.在此基础上,将携带p16基因与GM-CSF基因的腺病毒联合瘤体内注射,治疗预成性肿瘤,并对其免疫学作进一步探讨.首先,我们观察了腺病毒对小鼠肾癌Renca细胞的转染效率,证实了在MOI值为50,转染时间为12h时,能够达到75%～80%的转染效率,并获得目的基因p16蛋白的高效表达,因而作为本研究合适的基因转染条件. In this study, a close relationship with p16 in renal cell carcinoma in mice as a model, in vitro transfection of p16 gene-carrying adenovirus mouse renal cell carcinoma Renca cells observed p16 gene modified Renca cell biological characteristics of changes in the basis , The adenovirus carrying p16 gene and GM-CSF gene was injected into the tumor for the treatment of the preformed tumor, and its immunology was further explored.First, we observed the effect of adenovirus on Renca cell line It was confirmed that transfection efficiency of 75% -80% can be obtained at an MOI value of 50 and a transfection time of 12 h, and high expression of the target gene p16 protein was obtained. Therefore, as a suitable gene transfection condition in this study .