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本文介绍应用装有聚碳酸盐筛膜的培养小室分离从裂殖体释放出来的诺氏猴疟原虫裂殖子的方法。孔径3微米的碳酸盐筛膜能通过猴的正常红细胞和含有环型体及滋养体的红细胞,而保留90%以上的裂殖体。在开始后30分钟内,从培养小室的流出液中含有
This article describes a method for isolating merozoites of Plasmodium nidulans released from schizonts using a culture cell equipped with a polycarbonate sieve. A carbonate sieve membrane with a pore size of 3 μm can retain more than 90% of schizonts through the monkey’s normal erythrocytes and erythrocytes containing the annulus and trophozoites. Within 30 minutes after the start, it is contained in the effluent of the culture chamber