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为探讨孤儿核受体(Nurr1)在多巴胺能神经元损伤修复过程中对特异性表型酪氨酸羟化酶(TH)的影响,本研究用6-羟基多巴胺(6-OHDA)损伤大鼠一侧前脑内侧束(MFB)制备单侧帕金森病(PD)模型,分别应用Western blot和免疫组织化学技术观察不同时间点中脑黑质中Nurr1、TH蛋白表达的变化及其相关性。结果显示,Nurr1蛋白表达在术后1d损伤侧黑质中即明显下降,7d后开始逐渐上升,14d接近正常水平,28d明显升高;TH蛋白表达在术后1d损伤侧黑质中无明显变化,7d开始下降,14d达最低,28d开始上升。上述结果提示,在6-OHDA损伤黑质-纹状体通路后,Nurr1的表达先下降后上升,其表达变化早于TH,可能在PD模型大鼠的损伤修复中发挥重要作用。
To investigate the effect of orphan nuclear receptor (Nurr1) on the specific phenotype tyrosine hydroxylase (TH) during the repair of dopaminergic neurons, we used 6-hydroxydopamine (6-OHDA) One side of the medial forebrain bundle (MFB) was used to prepare the unilateral Parkinson’s disease (PD) model. Western blot and immunohistochemistry were used to observe the changes of Nurr1 and TH proteins in substantia nigra at different time points and their correlation. The results showed that the expression of Nurr1 protein decreased significantly in the lesion side after 1 day and began to increase gradually after 7 days and reached a normal level on the 14th day and increased significantly on the 28th day. The expression of TH protein had no significant change , 7d began to decline, 14d reached the lowest, 28d began to rise. The above results suggest that the expression of Nurr1 firstly descends and then rises, and its expression changes earlier than TH, which may play an important role in the injury repair of PD model rats after 6-OHDA damages the substantia nigra and striatum.