猪胰弹性酶(Porcine Pancreatic Elastase,简称PPE)是一种以丝氨酸为活性中心的肽链内切酶。近几年,PPE已被用于临床,治疗高脂血症和某些心血管疾病。但未见有关PPE纤溶作用性质方面的报道,本文从猪胰弹性酶原粉提纯PPE,并探讨其纤溶作用性质。 PPE原粉经DEAE-Sephadex A50、CMC柱层析纯化后,在SDS-聚丙烯酰胺凝胶电泳和等电聚焦电泳上呈一条区带,达到了电泳纯。冻干PPE纯品比活力平均127.1u Porcine Pancreatic Elastase (PPE) is an endopeptidase with serine as the active center. In recent years, PPE has been used clinically to treat hyperlipidemia and certain cardiovascular diseases. However, there was no report about the nature of PPE fibrinolysis. In this paper, purified PPE from porcine pancreatic elastase powder and its fibrinolytic properties were discussed. After the original powder of PPE was purified by DEAE-Sephadex A50 and CMC column chromatography, a band was observed on SDS-polyacrylamide gel electrophoresis and isoelectric focusing electrophoresis, reaching the electrophoresis purity. Lyophilized PPE pure than the average activity of 127.1u