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目的 探讨α－ 转化生长因子（ ＴＧＦα） 及其受体———表皮生长因子受体（ＥＧＦＲ） 在人原发性肝细胞肝癌（ ＨＣＣ） 组织蛋白和分子水平上的表达及意义．方法 运用免疫组织化学和原位杂交方法对７０ 例ＨＣＣ 进行研究，并采用５ 例正常人类肝脏做为对照．结果 ①７０ 例ＨＣＣ 中ＴＧＦ－ α的阳性率为７４－３ ％ ；ＥＧＦＲ 的阳性率为３８－６ ％ ． ②ＴＧＦα与 ＨＣＣ 的分化程度有关（ Ｐ ＜０－０５） ，在高分化ＨＣＣ 中ＴＧＦα的阳性率明显高于低分化的ＨＣＣ． ③有９３－６ ％ 的ＥＧＦＲ 阳性组织同时表现为ＴＧＦα阳性，二者的表达在 ＨＣＣ 组织中呈显著正相关（ Ｐ＜ ０－０５ ，γ＝ ０－４０） ． ④在 ＨＣＣ 癌旁组织中，ＴＧＦ － α的阳性率为８８－１ ％ ，ＥＧＦＲ 的阳性率为５４－２ ％ ，与其在ＨＣＣ 组织中相比，无显著差异（ Ｐ ＞ ０－０５ ） ，ＴＧＦα与 ＥＧＦＲ 呈显著正相关（ Ｐ＜ ０－０５ ，γ＝ ０－３５） ． ⑤ＴＧＦα与ＥＧＦＲ 的原位杂交结果略高于免疫组织化学结果，但二者之间无显著差异（ Ｐ＞ ０－０５） ．结论 ＴＧＦα，ＥＧＦＲ 可能与ＨＣＣ 的发生有关，在ＨＣＣ 发生中可能存在ＴＧＦα的自?
Objective To investigate the expression and significance of α-transforming growth factor (TGF-α) and its receptor, epidermal growth factor receptor (EGFR), in human primary hepatocellular carcinoma (HCC) tissue protein and molecular level. . Methods 70 cases of HCC were studied by immunohistochemistry and in situ hybridization. Five normal human livers were used as controls. Results The positive rate of TGF-α in 74 cases of HCC was 74-3%; the positive rate of EGF-R was 38-6 %. 2 The level of TGFα was related to the differentiation of HCC (P<0-05). The positive rate of TGFα in highly differentiated HCC was significantly higher than that of poorly differentiated HCC. 3 93% to 60% of EGF-R positive tissues were positive for TGF-α at the same time. There was a significant positive correlation between the two expressions in HCC tissues (P<0-05, γ=0-40). 4 In HCC adjacent tissues, the positive rate of TGF-α was 88-1 %, and the positive rate of EGF-R was 54-2 %. There was no significant difference compared with HCC tissue (P > 0-05). There was a significant positive correlation between TGF-α and EGF-R (P<0-05, γ=0-35). 5 The results of in situ hybridization of TGF-α and EGF R were slightly higher than those of immunohistochemistry, but there was no significant difference between them (P 0-05). Conclusions TGFα and EGFR may be related to the occurrence of HCC. TGFα autophagy may exist in HCC.