目的:体外观察塞来昔布对人膀胱癌T24细胞株的抑制作用,并探讨其可能的机制。方法:采用MTT法检测塞来昔布对T24细胞的生长抑制作用;采用Hoechst 33258染色观察细胞凋亡形态;采用Western印迹法检测塞来昔布作用前后T24细胞COX-2蛋白的表达变化;采用RT-PCR法检测塞来昔布作用前后T24细胞Bcl-2及Bax基因的表达变化。结果:塞来昔布(≥100μmol/L)作用24 h及48 h后能明显抑制T24细胞的生长并诱导凋亡;塞来昔布100μmol/L作用24 h及48 h后,T24细胞COX-2蛋白表达量下降,Bcl-2基因表达亦下调,而Bax基因在塞来昔布作用24 h后变化不明显,48 h后表达上调。结论:塞来昔布能抑制T24细胞增殖并诱导其凋亡,可能与下调Bcl-2、上调Bax基因表达有关。 Objective: To observe the inhibitory effect of celecoxib on human bladder cancer cell line T24 in vitro and to explore its possible mechanism. Methods: MTT assay was used to test the inhibitory effect of celecoxib on T24 cells. Hoechst 33258 staining was used to observe the apoptotic morphology of T24 cells. Western blotting was used to detect the expression of COX-2 protein in T24 cells before and after celecoxib treatment. The changes of Bcl-2 and Bax gene expression in T24 cells before and after celecoxib treatment were detected by RT-PCR. Results: Celecoxib (≥100μmol / L) could significantly inhibit the growth of T24 cells and induce apoptosis at 24 h and 48 h. After treated with 100 μmol / L of celecoxib for 24 h and 48 h, the COX- 2 protein expression decreased, Bcl-2 gene expression was down-regulated, while Bax gene in celecoxib 24 h after the change was not obvious, up-regulated after 48 h. Conclusion: Celecoxib can inhibit T24 cell proliferation and induce its apoptosis, which may be related to down-regulating Bcl-2 and up-regulating Bax gene expression.