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以3个普通小麦品种富可(Fuhuko)、中国春(Chinese Spring)及小偃759和毛穗赖草杂交,发现三个品种都可与毛穗赖草杂交,其中Fuhuko×L.paboanus平均结实率高达17.6％,杂种只有发育不全的幼胚而无胚乳。对杂种幼胚在N_6+1—2mg/11BA+0.5mgGA_3或MS(其中NH_4NO_3含量降低一半)附加1mg/1IBA的培养基上进行保姆培养,部分幼胚发育成完整的小植株,大部分幼胚死亡,并且在MS(1/2NH_4NO_3)培养基上,两个胚(Fuhuko×L.psboanus)形成质量很差的小愈伤组织,对其进行改良培养,建立了两个杂种胚性无性细胞系(一个生长很快,另一个相对较慢)。杂种愈伤组织在附加1mg/1IBA的MS或N_6培养基以及附加(0.5mg NAA+0.5mgKT)/1的MS(1/2NH_4NO_3)分化培养基上均可高频率产生再生植株。同时发现:1.将MS培养基中硝酸铵的含量降低一半,可显著提高植株再生频率;2.降低分化培养基中生长素(如IBA,NAA)的含量,加入少量的细胞激动素(如0.5 mg/1KT)可促使大量胚状体萌发,产生正常植株,使绿苗中90.0％以上的植株来自胚状体发生途径。细胞学观察表明:幼胚直接成苗的杂种植株体细胞染色体数很稳定,2n=63+1B,和预期结果相符;而杂种愈伤组组再生植株染色体数极不稳定,不同株间染色体数不同,即使同一根尖中不同的细胞染色体数也有很大差异,造成这种
Three common wheat cultivars, Fuhuko, Chinese Spring and Xiaoyan 759 were crossed with Leymus, to find that all three cultivars were crossed with Leymus secalinus, in which Fuhuko × L.paboanus was averagely strong The rate of up to 17.6%, hybrid only hypoplastic embryos without endosperm. The hybrid immature embryos were nursery on medium supplemented with 1mg / 1IBA of N_6 + 1-2mg / 11BA + 0.5mgGA_3 or MS (in which the content of NH_4NO_3 decreased by half), some immature embryos developed into complete plantlets, most of the immature embryos Died, and two embryos (Fuhuko × L.psboanus) formed poor quality small callus on MS (1/2 NH 4 NO 3) medium. The two calluses were subcultured to establish two hybrid embryonic clonal lines (One growing fast and the other relatively slow). Hybrid callus produced regenerated plants with high frequency on MS or N_6 medium supplemented with 1 mg / 1 IBA and MS (1/2 NH_4NO_3) differentiation medium supplemented with (0.5 mg NAA + 0.5 mgKT) / 1. At the same time, it was found that: 1. Reducing the content of ammonium nitrate in MS medium by half could significantly increase the frequency of plant regeneration; 2.Decreasing the content of auxin (such as IBA, NAA) in differentiation medium, adding a small amount of cytokinin 0.5 mg / 1KT) promoted the germination of a large number of embryoid bodies and produced normal plants. More than 90.0% of the plants in the green shoots came from the embryogenesis pathway. Cytological observations showed that the number of somatic chromosomes in hybrid plants of immature embryos was very stable, 2n = 63 + 1B, which was consistent with the expected results. However, the chromosome numbers of the regenerated plants in the hybrid callus group were extremely unstable. The number of chromosomes Different, even if the same root tips of different cell chromosomes are also very different, resulting in this