目的:体外研究内毒素对巨噬细胞炎症蛋白-2γ(MIP-2γ)表达的作用。方法:选用小鼠单核-巨噬细胞株RAW264.7及原代培养的BALB/c小鼠肾脏细胞用LPS刺激,以实时荧光定量RT-PCR检测不同时间点MIP-2γmRNA表达水平的变化。结果:RAW264.7细胞在LPS刺激后2 h,MIP-2γ.mRNA表达水平快速到达峰值(较正常水平增加约7倍),以后缓慢下降,至刺激后16 h基本恢复正常水平。原代培养肾脏细胞经LPS刺激后12 h,MIP-2γmRNA表达水平增长约50倍。结论:LPS在体外可明显诱导单核-巨噬细胞和肾脏细胞MIP-2γ mRNA的表达,提示MIP-2γ参与了炎症过程。 OBJECTIVE: To investigate the effect of endotoxin on the expression of macrophage inflammatory protein-2γ (MIP-2γ) in vitro. Methods: Mouse mononuclear macrophage cell line RAW264.7 and primary cultured BALB / c mouse kidney cells were stimulated with LPS, and the changes of MIP-2γ mRNA expression at different time points were detected by real-time fluorescence quantitative RT-PCR. Results: The expression of MIP-2γ.mRNA rapidly reached the peak at 2 h after LPS stimulation in RAW264.7 cells (about 7-fold increase from the normal level), then decreased slowly and returned to normal level 16 h after stimulation. At 12 h after primary cultured kidney cells were stimulated with LPS, MIP-2γ mRNA expression increased about 50-fold. CONCLUSION: LPS can obviously induce the expression of MIP-2γ mRNA in monocytes-macrophages and kidney cells in vitro, suggesting that MIP-2γ is involved in the inflammatory process.