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本研究采用两个小麦赤霉病菌—禾谷镰孢菌株Ｆ１５（强致病力菌株）和Ｈ２８（弱致病力菌株），经过菌丝培养、冷冻、匀浆、醋酸缓冲液淋洗、硼酸缓冲液初提、透析、乙醚萃取、冷冻干燥等一系列提取过程，得到了菌丝细胞壁提取物（ＨＣＷ）。采用考马斯亮兰法和酚－硫酸法分别测得两菌株ＨＣＷ中的蛋白质和碳水化合物的含量分别为１５％和７５％（Ｈ２８）和２０％和７０％（Ｆ１５）。用过碘酸和品红硫酸盐等进行特异性染色结果表明，ＨＣＷ中还含有一定量的糖蛋白。ＨＣＷ中的活性成分能被截留分子量大于３０ｋＤ的透析膜截留，在ＤＥＡＥ５２柱上很好地吸附和洗脱，表明ＨＣＷ中的活性组分是一种分子量较大的物质。经热处理、蛋白酶处理和过碘酸氧化处理后的生物活性测定结果分析表明，ＨＣＷ的活性组分可能为糖蛋白中的碳水化合物部分。
In this study, two wheat Fusarium graminearum strains F15 (virulent virulence strain) and H28 (weak pathogenic strain) were used in this study. After mycelial culture, freezing, homogenization, washing with acetic acid buffer, Buffer initial extraction, dialysis, ether extraction, freeze-drying and a series of extraction process, obtained mycelial cell wall extract (HCW). The protein and carbohydrate contents of HCW in both strains were 15% and 75% (H28) and 20% and 70% (F15), respectively, using Coomassie brilliant blue and phenol-sulfuric acid methods. Specific staining with periodic acid and magenta sulfate showed that HCW also contained certain amount of glycoprotein. The active ingredient in HCW can be retained by the dialysis membrane with molecular weight cut-off of more than 30kD and adsorbed and eluted well on DEAE52 column, indicating that the active ingredient in HCW is a kind of substance with larger molecular weight. Analysis of bioassay results after heat treatment, protease treatment and periodic acid oxidation showed that the active component of HCW may be the carbohydrate moiety in glycoproteins.