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目的:采用Alamar blue法及MTT法观察波罗蜜属药用植物单体化合物(ACR-2、ACR-3)对人肝癌细胞(SMMC-7721)和胃腺癌细胞(SGC-7901)增殖的影响。方法:用Alamar blue法和传统MTT法检测ACR-2、ACR-3对细胞SMMC-7721、SGC-7901的增殖抑制作用。结果:ACR-2作用于细胞SMMC-7721和细胞SGC-7901的IC50(mmol/L)分别为0.013和0.059;ACR-3作用于细胞SMMC-7721和细胞SGC-7901的IC50(mmol/L)分别为0.029和0.169。其中ACR-2、ACR-3对细胞SMMC-7721的增殖抑制作用最强,对细胞SGC-7901也表现出较强的增殖抑制作用。时量曲线检测结果显示:ACR-2、ACR-3对细胞SMMC-7721、SGC-7901的增殖抑制作用具有时间和剂量依赖性,其增殖抑制作用以48h时最强。结论:波罗蜜属药用植物单体化合物ACR-2、ACR-3对人肝癌细胞SMMC-7721、胃腺癌细胞SGC-7901有明显的增殖抑制作用,且有时间和剂量依赖性。
OBJECTIVE: To observe the effect of ACR-2, ACR-3 on the proliferation of human hepatoma cells (SMMC-7721) and gastric adenocarcinoma cells (SGC-7901) by Alamar blue method and MTT assay. METHODS: Alamar blue and traditional MTT assays were used to detect the inhibitory effects of ACR-2 and ACR-3 on the proliferation of SMMC-7721 and SGC-7901 cells. RESULTS: The IC50 (mmol/L) of ACR-2 on SMMC-7721 and SGC-7901 cells was 0.013 and 0.059, respectively; the IC50 of ACR-3 on SMMC-7721 cells and SGC-7901 cells (mmol/L). They are 0.029 and 0.169 respectively. Among them, ACR-2 and ACR-3 had the strongest inhibitory effect on the proliferation of SMMC-7721 cell, and also showed a strong inhibitory effect on cell SGC-7901. The results of time-volume curve test showed that the proliferation inhibitory effects of ACR-2 and ACR-3 on cells SMMC-7721 and SGC-7901 were time-and dose-dependent, and their inhibition of proliferation was the strongest at 48 hours. Conclusion: The plant monomer compounds ACR-2 and ACR-3 of Plutella spp. have obvious inhibitory effects on the proliferation of human hepatocellular carcinoma cells SMMC-7721 and gastric adenocarcinoma cells SGC-7901 in a time- and dose-dependent manner.