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用不同浓度和pH的磷酸缓冲液喷施菜豆叶片,然后低温处理离体叶片,测定叶片的相对含水量、水势、渗透势和低温下的膜透性,以探讨磷酸缓冲液喷施菜豆叶片的适宜浓度,pH及其提高耐冻的生理效应。主要试验结果如下:1.0.1M、pH6.45的磷酸缓冲液可增加菜豆叶片的耐冻力。但这种增强效应是暂时性的。2.处理菜豆叶片24小时后,模拟霜冻处理幼苗的成活率比对照高29.0％。离体叶片在处理后24小时耐冻力最强,低温伤害的临界温度下降0.8℃左右。但随着处理后时间的延长,耐冻力减弱,到120小时,叶片质膜透性己恢复到对照水平。3.菜豆处理后,其叶片相对含水量下降,水势降低,渗透势升高;菜豆叶片脱水2—4％,可以增加叶片的耐冻力。
The foliar application of different concentrations and pH of Phyllostachys pubescens leaves was sprayed and the detached leaves were treated at low temperature. The relative water content, water potential, osmotic potential and membrane permeability at low temperature were measured to investigate the effects of phosphate buffered saline Appropriate concentration, pH, and their physiological effects to increase freeze tolerance. The main test results are as follows: 1.0.1M, pH6.45 phosphate buffer can increase the resistance of the bean leaf freeze resistance. However, this enhancement is temporary. After treatment for 24 hours, the survival rate of simulated frost-treated seedlings was 29.0% higher than that of the control. Detached leaves in the treatment of 24 hours after the strongest frost resistance, the critical temperature of low temperature injury decreased about 0.8 ℃. However, with the prolongation of the treatment time, the frost resistance was weakened. By 120 hours, the leaf plasma membrane permeability had returned to the control level. 3. Beans treated, the relative water content of leaves decreased, water potential decreased, osmotic potential increased; 2-4% dehydration of bean leaves, can increase the leaf resistance to freezing.