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为了探讨人乳头状瘤病毒(HPV)感染和P~(53)基因突变与喉不典型增生病变癌变的关系,应用多聚酶链反应(PCR)方法对18例喉不典型增生病变标本中HPV16-18和P~(58)基因突变进行了检测,并作了临床随访。结果发现18例中HPV16～18阳性9例,P~(53)阳性5例,HPV16-18和P~(53)两者均阳性者5例,HPV16-18阳性P~(53)阴性者4例,HPV16-18和P~(53)两者均阴性者9例。有10例在随访中发展为喉鳞状细胞癌,其中HPV16-18和P~(53)均阳性的5例都发生了癌变,HPV16-18阳性PP~(53)阴性的4例中3例发生了癌变,而HPV16-18和P~(53)均阴性的9例中仅2例发生癌变。结果提示喉不典型增生病例如HPV16-18和P~(53)均阳性者有较高的癌变率,对这部分病人临床上必须密切随访。
In order to investigate the relationship between human papillomavirus (HPV) infection and P53 mutation and the carcinogenesis of the atypical hyperplasia of the larynx, polymerase chain reaction (PCR) was used to detect the expression of HPV16-18 in 18 cases of atypical hyperplasia of the larynx And P ~ (58) gene mutations were detected and made a clinical follow-up. The results showed that in 18 cases, HPV16-18 was positive in 9 cases, P53 positive in 5 cases, HPV16-18 and P53 were both positive in 5 cases, HPV16-18 positive in 53 negative 4 Cases, HPV16-18 and P ~ (53) were negative in both 9 cases. Ten patients developed laryngeal squamous cell carcinoma at follow-up, of which 5 cases were positive for HPV16-18 and P53, and 3 cases were negative for HPV16-18-positive PP 53 Carcinogenesis occurred, but only 2 of 9 cases with HPV16-18 and P53 negative were cancerous. The results suggest that atypical hyperplasia of the throat, such as HPV16-18 and P53 positive were higher rates of cancer, this part of the patients clinically close follow-up.