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目的 :探讨氯化甲基汞 ( MMC)对发育大鼠小脑组织转录因子 CREB DNA结合活性的影响。方法 :将妊娠大鼠于妊娠 7～ 1 0 d连续 4d每日灌胃给予氯化甲基汞 4mg· kg-1,取生后 1、3、7、1 4d大鼠小脑组织提取核蛋白。采用凝胶移位法观察 MMC对发育阶段大鼠小脑转录因子CREB DNA结合活性的影响。结果 :对照组和实验组各发育阶段小脑组织 CREB在凝胶移位电泳中均呈现两条迟滞带 ;对照组和实验组 P1 - 7大鼠幼仔小脑 CREB DNA结合活性随生后发育时间延长呈下降趋势 ;实验组大鼠幼仔小脑 CREB DNA结合活性均高于相应对照组。结论 :CREB参与大鼠脑发育的调节 ;甲基汞所致发育脑组织损伤可能与其引起 CREB DNA结合活性升高有关。
Objective: To investigate the effect of methylmercury chloride (MMC) on CREB DNA binding activity of cerebrum in developing cerebellum. Methods: Pregnant rats were administered with 4 mg · kg-1 methylmercury methyl mercury (GMA) orally daily for 4 days on day 7 to day 10 of gestation, and the cerebellum was extracted from rats’ cerebellum at 1, 3, 7 and 14 days after birth. The effect of MMC on CREB DNA binding activity of rat cerebellum during cerebellum development was observed by gel shift method. Results: The CREB of cerebellum in control group and experiment group showed two hysteresis bands in gel shift electrophoresis. The CREB DNA binding activity in cerebellum of P1 - 7 rats in control group and experimental group was prolonged with time after birth Showing a downward trend; CREB DNA binding activity in the cerebellum of experimental group rats were higher than the corresponding control group. CONCLUSIONS: CREB is involved in the regulation of brain development in rats. Methylmercury-induced brain injury may be related to the increased CREB DNA binding activity.