论文部分内容阅读
观察氟铝联合对体外培养人胚成骨细胞毒作用。方法：采用无血清体外细胞培养方法，将成骨细胞分成对照组、低铝组、高铝组、低铝低氟组、高铝低氟组及高铝高氟组共６组，以光镜和电镜观察其形态学变化。结果：光镜观察，高铝组和高铝加氟组细胞存活数均明显低于对照组、低铝组和低铝低氟组（ Ｐ＜ ０． ０５）。高铝组细胞变性坏死率明显高于对照组、低铝组和低铝低氟组（Ｐ＜０．０５）。在高铝组加入不同剂量的氟可明显降低细胞变性坏死率，但仍明显高于对照组、低铝组及低铝低氟组（ Ｐ＜ ０． ０５），而明显低于高铝组（ Ｐ＜ ０． ０５）；电镜观察，高铝组和高铝加氟组细胞核膜迂曲肿胀，线粒体膜结构不清楚，胞质内含有大量次级和终末溶酶体，多数为髓鞘样结构，而低铝组和低铝低氟组变化轻微。
To observe the toxic effect of fluoro-aluminum on cultured human embryo osteoblasts in vitro. Methods: The osteoblasts were divided into six groups: control group, low aluminum group, high aluminum group, low aluminum low fluoride group, high aluminum low fluoride group and high aluminum fluoride group by serum-free in vitro cell culture method. Electron microscope observation of the morphological changes. Results: The number of cells in high-aluminum group and high-fluoride group was significantly lower than that in control group, low-aluminum group and low-aluminum-low fluoride group (P <0.05). The rate of cell degeneration and necrosis in high-aluminum group was significantly higher than that in control group, low-aluminum group and low-aluminum-low fluoride group (P <0.05). The addition of different doses of fluoride in the high aluminum group could significantly reduce the rate of cell degeneration and necrosis, but still significantly higher than the control group, low aluminum group and low aluminum fluoride group (P <0.05), but significantly lower than the high aluminum group P <0.05). Electron microscopy showed that tortuous swelling of the nuclear membrane in the high-aluminum group and the high-aluminum-fluoride group resulted in unclear mitochondrial membrane structure and a large amount of secondary and terminal lysosomes in the cytoplasm, most of which were myelin-like structures , While the changes of low aluminum group and low aluminum low fluorine group changed slightly.