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鉴于疟疾血清学试验使用的同种人体疟原虫抗原的来源困难及使用体外培养的恶性疟原虫作抗原的研究报告尚少。本文采用体外培养的恶性疟原虫和夜猴体内感染的恶性疟原虫分别制备抗原,通过间接荧光抗体(IFA)及间接血凝(IHA)试验进行比较研究。试验所用的恶性疟原虫对氯喹是敏感的,用RPMI 1640培养基在体外培养保存。另以夜猴体内繁殖的恶性疟原虫制备抗原(称CDC抗原)作对照,以IHA及IFA试验同时检测14份血清,其中7份为已知阳性血清,3份已知阴性血清,另4份血清有否疟疾史不明。
In view of the difficulties of origin of the same kind of human Plasmodium antigen used in malaria serological tests and the use of P. falciparum in vitro as antigens, few reports have been reported. In this study, anti-Plasmodium falciparum and Plasmodium falciparum were used to prepare antigens in vitro and compared by indirect fluorescent antibody (IFA) and indirect hemagglutination (IHA). Plasmodium falciparum was sensitive to chloroquine and was maintained in vitro in RPMI 1640 medium. In addition, P. falciparum was used to prepare antigens (called CDC antigens) in control of P. falciparum. 14 serum samples were simultaneously tested by IHA and IFA tests, of which 7 were known positive sera, 3 were negative and 4 were negative Serum history of malaria is unknown.