论文部分内容阅读
树突状细胞研究热潮的掀起,源于发现了强大的抗原递呈功能.其抗瘤的思路,旨在补偿肿瘤逃逸机制中缺失的抗原信号和共刺激因子,体外扩增方法的成熟已展现了它良好的应用前景.HSV-tk/GCV系统基因疗法的关键,在于它直接杀伤或通过旁观者效应杀伤的肿瘤细胞,能使机体产生肿瘤特异性免疫.将树突状细胞与MCF-7/tk瘤苗联合应用,是集免疫和基因疗法优势的综合抗癌途径.本课题依据树突状细胞在抗原摄取、递呈上的特性,结合HSV-tk/GCV系统的旁观者效应能产生凋亡的特点,将两者联合应用,目的在于使其既能发挥自杀基因的瘤苗“接种作用”,又能使DC吞噬凋亡小体后进一步激发T细胞增殖,诱导特异性CTL生成.
The upsurge of research on dendritic cells stems from the discovery of a powerful antigen presentation function. Its anti-tumor strategy is designed to compensate for missing antigenic signals and co-stimulatory factors in tumor escape mechanisms. The maturation of in vitro amplification methods has been demonstrated. It has a good prospect of application. The key to HSV-tk/GCV system gene therapy lies in its direct killing or killing of tumor cells by the bystander effect, enabling the body to produce tumor-specific immunity. The dendritic cells and MCF-7 The combined use of /tk tumor vaccines is a comprehensive anti-cancer approach that combines the advantages of immune and gene therapy. This topic is based on the characteristics of dendritic cells in antigen uptake and presentation, combined with the bystander effect of the HSV-tk/GCV system. The characteristics of apoptosis, the combination of the two, the purpose is to make it not only can exert the “inoculation effect” of the tumor vaccine of suicide gene, but also enable DC to phagocytose apoptotic bodies to further stimulate the proliferation of T cells and induce the generation of specific CTL.