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近几年来,许多不同类型的自动化样品制备技术取得较大进展,使用实验室机器人便是其中之一。人工操作步骤(Ⅰ法):将200μl 血浆置于5ml 聚丙烯试管中,加入水、内标液和饱和(NH_4)_2SO_4溶液各200μl,涡旋混合10s,再加入1.6ml 氯仿-异丙醇(95∶5),涡旋混合80s,离心10min,将有机相转移至自动取样器小瓶中,进样20μl 于 LichrosorbSi-60柱进行测定。流动相的组成为:氯仿(1325ml)、正庚烷(950ml)、甲醇(225ml)、四氢
In recent years, many different types of automated sample preparation technology have made great progress, the use of laboratory robots is one of them. Manually operated procedure (method I): Place 200μl of plasma into 5ml polypropylene tube, add 200μl of water, internal standard solution and saturated (NH_4) _2SO_4 solution, vortex and mix for 10s, then add 1.6ml of chloroform-isopropanol 95: 5), vortex mixed for 80s, centrifuged for 10 min, the organic phase was transferred to an autosampler vial and 20 [mu] l was injected on a Lichrosorb Si-60 column. The mobile phase consisted of chloroform (1325 ml), n-heptane (950 ml), methanol (225 ml), tetrahydro