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为了探明荔枝漆酶基因LcLac的表达与果皮褐变之间的关系,通过筛选‘妃子笑’荔枝果皮cDNA文库和3′-RACE技术,克隆获得了荔枝LcLac全长cDNA序列(EU527187.1)。该序列全长1779bp,5′-UTR长26bp,3′-UTR长52bp,含一个1701bp的完整开放阅读框,编码含566个氨基酸残基的多肽。该氨基酸残基序列与欧亚槭树等物种的漆酶蛋白相似性较高。荧光定量PCR结果表明,LcLac在荔枝花中表达量最高,果肉中表达量最低。在采后贮藏前期,随LcLac表达量上升果皮褐变指数升高,且褐变指数较高的‘妃子笑’果皮中LcLac表达量高于褐变指数低的‘紫娘喜’果皮。贮藏中后期严重褐变果皮中LcLac表达量比贮藏前期低。采后贮藏前期果皮中LcLac的上调表达可能对其褐变起促进作用。
In order to find out the relationship between LcLac expression and pericarp browning, the full-length LcLac cDNA sequence (EU527187.1) was cloned by screening litchi peel cDNA library and 3’-RACE technology. . The full-length of this sequence is 1779bp, 26bp in 5’-UTR and 52bp in 3’-UTR. It contains a complete open reading frame of 1701bp and encodes a polypeptide of 566 amino acid residues. The amino acid residue sequence and Eurasian maple and other species of laccase protein similarity is high. Fluorescent quantitative PCR results showed that LcLac had the highest expression in litchi flowers and the lowest expression in pulp. In early prophase storage, the expression of LcLac in peel increased with the increase of LcLac expression and peel browning index was higher than that of ’Zi Niang hi’ peel with low browning index. The expression of LcLac in the late-period peanut husk was lower than that in the early stage of storage. The up-regulated expression of LcLac in the pericarp of post-harvest storage may promote its browning.