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报道了分离提纯自古尼虫草(Cordyceps gunii)一个单子囊孢子的无性古尼拟青霉(Paecilo—myces gunii)5—19菌株,菌丝培养物镇痛组分的基本理化性质和菌丝不同培养条件对其药理作用的影响。结果表明:虫草菌丝的镇痛组分是水溶性的;镇痛组分最佳提取溶剂为水,最佳提取方法为热提取法;古尼虫草镇痛组分对冰醋酸所致小鼠扭体抑制率在剂量0.05 g/g(生药/小鼠)范围与剂量成正相关;在所试pH值范围内(pH1～10),其镇痛效果无明显变化。当pH>11时,镇痛作用强度下降。镇痛组分有较好的热稳定性。在100℃加热1 h,它对冰醋酸所致的小鼠扭体抑制率仍为100％;培养基组成和培养温度在所试条件下,不仅影响菌丝的生物量,而且影响菌丝浸出物的镇痛效果。
Reported the isolation and purification of Paecilo-myces gunii strain 5-19 from a single ascosporum of Cordyceps gunii. The basic physiochemical properties of the analgesic component of mycelial cultures and different cultures of mycelium were reported. The effect of conditions on its pharmacological effects. The results showed that the analgesic component of Cordyceps mycelium was water-soluble; the best extraction solvent for analgesic components was water, and the best extraction method was hot extraction; the Cordyceps militaris analgesic component against acetic acid-induced mice The writhing inhibition rate was positively correlated with the dose in the dose range of 0.05 g/g (crude drug/mouse); in the tested pH range (pH 1 to 10), the analgesic effect did not change significantly. When pH>11, the intensity of analgesic effect decreased. Analgesic components have better thermal stability. When heated at 100°C for 1 h, the inhibition rate of writhing caused by glacial acetic acid was still 100%. Under the tested conditions, the medium composition and culture temperature not only affected the biomass of mycelia, but also affected mycelia leaching. The analgesic effect of the material.