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目的：了解人参皂甙（ＧＳ）对巨核系祖细胞（ＣＦＵ－Ｍｅｇ）的刺激增殖作用，比较ＧＳ与生长因子ＴＰＯ、ＩＬ－３对ＧＡＴＡ转录调控蛋白的诱导作用。方法：正常人和ＩＴＰ患者骨髓ＣＦＵ－Ｍｅｇ培养和ＧＳ刺激试验、电泳带移动阻滞试验（ＥＭＳＡ）、抗体胶移动试验（Ｓｕｐｅｒｓｈｉｆｔ ａｓｓａｙ）和蛋白免疫印迹法（Ｗｅｓｔｅｒｎ Ｂｌｏｔ）。结果：ＧＳ刺激正常ＣＦＵ－Ｍｅｇ增殖，呈剂量依赖的方式，血小板减少性紫癜（ＩＴＰ）患者的ＣＦＵ－Ｍｅｇ也对ＧＳ敏感。ＧＳ、ＴＰＯ和ＩＬ－３均能诱导生长因子依赖ＭＯ７ｅ细胞核内ＧＡＴＡ蛋白与ＤＮＡ结合的活性及使ＧＡＴＡ－２蛋白含量增加。ＧＳ＋ＴＰＯ或ＧＳ＋ＩＬ－３可协同地诱导ＧＡＴＡ蛋白活性进一步增高。结论：ＧＳ能够通过类生长因子和增强生长因子作用而刺激ＣＦＵ－Ｍｅｇ增殖，ＧＡＴＡ族转录因子，主要是ＧＡＴＡ－２参与造血细胞对ＧＳ反应的信号传递途径。
Objective: To investigate the effect of ginsenosides (GS) on the proliferation of megakaryocyte progenitor cells (CFU-Meg) and to compare the induction of GATA transcriptional regulatory proteins by GS and growth factors TPO and IL-3. METHODS: Bone marrow CFU-Meg culture and GS stimulation assays, electrophoretic mobility shift assay (EMSA), Supershift assay, and Western Blot were performed in normal human and ITP patients. Results: GS stimulated proliferation of normal CFU-Meg in a dose-dependent manner, and CFU-Meg in patients with thrombocytopenic purpura (ITP) was also sensitive to GS. GS, TPO and IL-3 all can induce the growth factor-dependent activity of GATA protein binding to DNA in the nucleus of MO7e and increase the GATA-2 protein content. GS+TPO or GS+IL-3 can synergistically induce further increase of GATA protein activity. Conclusion: GS can stimulate the proliferation of CFU-Meg through the action of growth factors and growth factors. GATA family transcription factor, mainly GATA-2, is involved in the signal transduction pathway of GS reaction in hematopoietic cells.