论文部分内容阅读
AIM To establish a cell culture system with long-termreplication of hepatitis C virus in vitro.METHODS Human hepatoma cell line 7721 was tested forits susceptibility to HCV by incubating with a serum from apatient with chronic hepatitis C.Cells and supernatantwere harvested at various phases during the culturingperiods.The presence of HCV RNA,the expression ofHCV antigens in cells and/or supernatant were examinedby RT-PCR,in situ hybridization and immunohisto-chemistry respectively.RESULTS The intracellular HCV RNA was first detectedon d2 after infection and then could be intermittentlydetected in both cells and supernatant over a period of atleast three months.The expression of HCV NS_3,CP_(10)antigens could be observed in cells.The fresh cells couldbe infected by supernatant from cultured infected cellsand the transmission of viral genome from HCV-infected7721 cells to PBMCs was also observed.CONCLUSION The hepatoma line 7721 is not onlysusceptible to HCV but also supports its long-termreplication in vitro.
AIM To establish a cell culture system with long-term replication of hepatitis C virus in vitro.METHODS Human hepatoma cell line 7721 was tested forits susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C.Cells and supernatantwere harvested at various fruits during The culturingperiods.The presence of HCV RNA, the expression ofHCV antigens in cells and/or supernatant were examined by RT-PCR,in situ hybridization and immunohisto-chemistry respectively.RESULTS The intracellular HCV RNA was first detectedon d2 after infection and then could could be intermittentlydetected In both cells and supernatant over a period of atleast three months.The expression of HCV NS_3,CP_(10)antigens could be observed in cells.The fresh cells couldbe infected by supernatant from cultured infected cellsand the transmission of viral genome from HCV-infected7721 Cells to PBMCs was also observed.CONCLUSION The hepatoma line 7721 is not onlysusceptible to HCV but also supports its long-t Ermreplication in vitro.