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两性霉素Ｂ（ＡＭＢ）常用于内脏或全身真菌感染的治疗。由于ＡＭＢ对肝、肾等有较大毒性，应监测血浆中ＡＭＢ的浓度，以便调整其用量。我们采用高效液相色谱法（ＨＰＬＣ），在μ－ＢｏｎｄａｐａｋＣ１８柱（３．９ｍｍ×３００ｍｍ，１０μｍ）上，以０．０５ｍｏｌ／ＬＥＤＴＡ－２Ｎａ溶液－乙腈（１∶１）为流动相；流速为１．４ｍｌ／ｍｉｎ；检测波长为４０５ｎｍ，测定了血浆、脑脊液中ＡＭＢ浓度。ＡＭＢ血浆中最小检出量为０．０２μｇ／ｍｌ。血浆中ＡＭＢ提取率＞８７％。日内精密度在５．０１％～６．２８％之间，日间精密度血样＜７．８６％，ＣＳＦ＜５．９８％。血浆中ＡＭＢ浓度在０．０５～２．０μｇ／ｍｌ范围内有良好线性关系。方法回收率为９９．０４％±３．９０％。该法用于１例曲霉菌全身感染者ＡＭＢ血药浓度测定，为制定给药方案提供了依据。
Amphotericin B (AMB) is commonly used in the treatment of visceral or systemic fungal infections. As AMB on the liver, kidney and other more toxic, should monitor the concentration of AMB in plasma in order to adjust its dosage. We used high performance liquid chromatography (HPLC) on μ-Bondapak C18 column (3.9mm × 300mm, 10μm) with 0.05mol / LEDTA-2Na solution-acetonitrile .4ml / min; detection wavelength of 405nm, determination of plasma, cerebrospinal fluid AMB concentration. The minimum detectable amount of AMB plasma is 0.02 μg / ml. AMB extraction in plasma> 87%. Intra-day precision was between 5.01% and 6.28%, daytime precision blood samples <7.86% and CSF <5.98%. AMB concentrations in plasma ranged from 0.05 to 2.0 μg / ml and had a good linear relationship. The recovery rate was 99.04% ± 3.90%. The method was used to determine the blood concentration of AMB in 1 case of Aspergillus infection and provided the basis for formulating the drug delivery plan.