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根据茶树子叶柄、叶柄断面离腋芽的距离,将外植体依次分为C_1、C_2、C_3和L_1、L_2、L_3.通过上述外植体和不同培养基的研究结果表明:子叶柄外植体在MT_1和MT_3培养基上培养,其不定芽及总分化率是C_1>C_2>C_3;体细胞胚的分化率是C_3>C_2>C_1.叶柄外值体只有L_1在MT_3培养基上培养分化了芽,MT_1培养基的胚性愈伤组织诱导率比MT_3培养基高,其体细胞胚、不定芽的分化率是L_1>L_2、L_3.经挑选的胚性愈伤组织在MT_1和MT_2培养基上交叉培养,保持分化能力在15个月以上.体细胞胚、不定芽都能在MT_4培养基上增殖.
The explants were divided into C_1, C_2, C_3 and L_1, L_2, L_3 according to the distance between the axillary buds and the cross-section of the petioles of the tea plants.The results of the above explants and different media showed that the explants In MT1 and MT_3 medium, the adventitious buds and total differentiation rate were C_1> C_2> C_3, and the differentiation rate of somatic embryos was C_3> C_2> C_1. Only L_1 of petiole was differentiated in MT_3 medium The induction rate of embryogenic callus in MT1 medium was higher than that in MT3 medium, and the differentiation rate of somatic embryos and adventitious buds was L_1> L_2, L_3. The embryogenic callus was selected in MT1 and MT_2 medium On the cross-culture, to maintain the ability to differentiate in more than 15 months. Somatic embryos, adventitious buds can MT4 medium proliferation.