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目的研究全反式视黄酸(ATRA)对人乳腺癌MDA-MB-231细胞增殖及ATRA与X射线照射对视黄醇类X受体α(RXRα)表达水平的影响。方法 MTT法检测细胞增殖,Western blot检测RXRα表达水平。结果 ATRA浓度在100～1000 nmol/L,72 h可抑制细胞的增殖。观察不同时间100 nmol/L的ATRA作用,24 h内,促进MDA-MB-231细胞的增殖,而作用48 h后,可抑制细胞的增殖。ATRA在100～1000 nmol/L范围内可诱导RXRα的表达,且存在浓度依赖性。100 nmol/L的ATRA作用24 h即可诱导RXRα的表达,并且存在时间依赖性。X射线照射未能诱导RXRα的表达。结论 100～1000 nmol/L的ATRA作用48 h即可抑制MDA-MB-231细胞增殖,且其对MDA-MB-231细胞RXRα蛋白表达存在浓度和时间依赖性,为ATRA用于临床乳腺癌的治疗提供了理论基础,也提示RXRα可能是ATRA抑制乳腺癌细胞增殖的机制之一。
Objective To investigate the effect of all-trans retinoic acid (ATRA) on the proliferation of human breast cancer MDA-MB-231 cells and the effect of ATRA and X-ray irradiation on the expression of retinal X Receptor α (RXRα). Methods Cell proliferation was detected by MTT assay and RXRα expression by Western blot. Results ATRA concentrations of 100 ~ 1000 nmol / L, 72 h can inhibit cell proliferation. The effect of ATRA of 100 nmol / L at different time was observed. Within 24 h, the proliferation of MDA-MB-231 cells was observed. After 48 h, the proliferation of MDA-MB-231 cells was inhibited. ATRA can induce the expression of RXRα in the concentration range of 100 ~ 1000 nmol / L, and there is a concentration-dependent manner. The expression of RXRα was induced by 100 nmol / L ATRA for 24 h, and there was a time-dependent manner. X-ray irradiation failed to induce the expression of RXRα. CONCLUSION: ATRA of 100-1000 nmol / L can inhibit the proliferation of MDA-MB-231 cells at 48 h, and the concentration of RXRα protein in MDA-MB-231 cells is dependent on the concentration of ATRA in clinical breast cancer Treatment provides a theoretical basis, also suggested that RXRα may be ATRA inhibition of breast cancer cell proliferation mechanism.