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目的:观察脑源性神经营养因子(BDNF)对大鼠后足切割疼痛的影响。方法:采用纵行切割大鼠后足作为疼痛模型,运用免疫组织化学与免疫荧光双标记方法,观察大鼠后足切割后不同时间点(1-72hr)BDNF在相应节段背根神经节与脊髓内表达的变化。腹腔或鞘内注射BDNF抗体中和内源性BDNF后,以Von Frey尼龙纤维刺激后足行机械痛敏评价。结果:大鼠后足切割后1-24hr内,BDNF在切割侧L42-L5脊髓后角表达明增加,BDNF主要位于后角神经元内与神经末梢,星形胶质细胞与小胶质细胞内未见明显表达;在L42-L5背根神经节,BNDF免疫阳性细胞百分比在切割后1-24hr内也明显增加,增加的主要为大直经神经元;鞘内给予BDNF抗体可明显增加大鼠后足切割后的缩足阈值,而腹腔给予BDNF抗体对大鼠的缩足阈值影响较小。结论:BDNF参与了大鼠后足切割后机械痛敏的过程。
Objective: To observe the effect of brain derived neurotrophic factor (BDNF) on the hindpaw cutting pain in rats. Methods: The hindpaw of the rat was cut by longitudinal dissection, and the expression of BDNF at different time points (1-72hr) in the corresponding segmental dorsal root ganglion and the dorsal root ganglion were observed by immunohistochemistry and immunofluorescence double labeling. Spinal cord expression changes. After intraperitoneal or intrathecal injection of BDNF antibody neutralizes endogenous BDNF, mechanical mechanical allodynia was evaluated after Von Frey nylon fiber stimulation. Results: The expression of BDNF in the dorsal horn of L42-L5 spinal cord increased within 1-24 hours after the dorsal hind paw cutting. BDNF mainly localized in the posterior horn neurons and nerve endings, astrocytes and microglial cells No obvious expression was found in the L42-L5 dorsal root ganglion. The percentage of BNDF immunoreactive cells in the L42-L5 dorsal root ganglion was also significantly increased within 1-24 hours after cutting, the increase was mainly through the Dzengjing neurons; intrathecal administration of BDNF antibody significantly increased rats The contractile threshold after hind paw cut, while intraperitoneal administration of BDNF antibody had less effect on contractile threshold in rats. CONCLUSION: BDNF is involved in the process of mechanical hyperalgesia in rats after hindpaw cutting.