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本文报导了国内首例抗烟草花叶病毒(TMV)和黄瓜花叶病毒(CMV)的转基因双抗烟草新品系的选育方法,获得了具有抗TMV和CMV的双抗优质新品系。在新品系第4—6代的田间试验中,验证了其抗病毒特性的遗传稳定性;与对照品种NC89在农艺性状、品质和烘烤特性等方面进行比较,证明转基因抗病新品系除抗病性显著增强外,其它特性均略优于原NC89良种。1992年在河南省示范推广6667hm~2,转基因抗病新品系对烟草花叶病的相对控制效果为50%-70%,防病增产的经济效益显著。
This paper reports the breeding of the first new double transgenic tobaco tobaco resistant to TMV and cucumber mosaic virus (CMV) in China, and obtained a new high quality double strain resistant to TMV and CMV. The genetic stability of the antiviral trait was verified in the field trials of lines 4-6 in the new line. Comparing with the control line NC89 in terms of agronomic traits, quality and baking characteristics, Significantly increased disease, the other characteristics are slightly better than the original NC89 elite. Demonstration and promotion of 6667 hm ~ 2 in Henan Province in 1992, the relative control effect of new transgenic lines against tobacco mosaic disease was 50% -70%, and the economic benefits of disease prevention and yield increase were significant.