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报道了一种从真养产碱杆菌中提取聚－β－羟基丁酸酯的新方法，并将其与其它几种提取方法进行了比较。研究了各种提取方法对聚－β－羟基丁酸酯的纯度、提取率及摩尔质量的影响。结果表明，用有机溶剂在丙酮预处理后回流提取，聚－β－羟基丁酸酯的纯度达９７％以上，摩尔质量Ｍ＝３３０ｋｇ／ｍｏｌ左右，但提取率仅约７０％；用次氯酸钠提取时，纯度９０％，提取率８８％，但Ｍ只有２１６ｋｇ／ｍｏｌ，比起始菌体中的４０２ｋｇ／ｍｏｌ降低近一半；用分散的氯仿－次氯酸钠提取，聚－β－羟基丁酸酯纯度９７％，提取率８２％，Ｍ＝３１０ｋｇ／ｍｏｌ；酶法提取的纯度只有８５％；而用新发明的表面活性剂－络合剂法，聚－β－羟基丁酸酯纯度９８．７％，提取率达９３．３％，Ｍ＝３１６ｋｇ／ｍｏｌ．
A new method of extracting poly-β-hydroxybutyrate from Alcaligenes eutrophus was reported and compared with several other extraction methods. The effects of various extraction methods on the purity, extraction yield and molar mass of poly-β-hydroxybutyrate were studied. The results showed that the purity of poly-β-hydroxybutyrate was above 97% and the molar mass was about 330 kg / mol, but the extraction rate was only about 70%. After extracting with sodium hypochlorite , Purity of 90%, extraction rate of 88%, but M only 216kg / mol, 402kg / mol than the initial cell decreased by nearly half; with a dispersed chloroform - sodium hypochlorite extraction, poly-β-hydroxybutyrate purity of 97% , The extraction rate of 82%, M = 310kg / mol; enzymatic extraction purity of only 85%; with the newly invented surfactant - complexing agent, poly-β-hydroxybutyrate purity of 98.7% The rate of 93.3%, M = 316kg / mol.