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本实验将人血清LDL乙酰化后用真~(125)I标记做成放射性配基,对小鼠腹腔巨噬细胞表面的乙酰LDL受体进行了研究。结果表明:培养的小鼠腹腔巨噬细胞表面存在有能与乙酰LDL特异性,高亲和性和可饱和性结合的受体。它们不能识别天然LDL,主要功能是加速被结合的脂蛋白被巨噬细胞摄取并将其送至溶酶体进一步降解。该受体不受细胞内胆固醇含量的反馈调节。当巨噬细胞与乙酰LDL共同孵育时,可通过该受体大量摄取乙酰LDL使细胞蓄积胆固醇变成泡沫状细胞。经细胞内胆固醇含量测定、苏丹Ⅳ染色、透射电镜观察等方法鉴定,与动脉粥样硬化病灶中巨噬细胞源的泡沫细胞相似。
In our experiment, human LDL was acetylated and labeled with true ~ (125) I radiolabeled ligand. The acetylcholinesterase receptor on the surface of mouse peritoneal macrophages was studied. The results showed that the receptors on the surface of cultured mouse peritoneal macrophages could bind to acetylcholinesterase (LDL) specificity, high affinity and saturability. They do not recognize native LDL and their main function is to accelerate the uptake of bound lipoproteins by macrophages and their delivery to lysosomes for further degradation. The receptor is not regulated by the feedback of intracellular cholesterol content. When macrophages are co-incubated with acetyl-LDL, large amounts of acetyl-LDL can be taken through the receptor to turn the cells’ accumulated cholesterol into foamy cells. By the determination of intracellular cholesterol content, Sudan Ⅳ staining, transmission electron microscopy and other methods identified, and macrophage-derived foam cells in atherosclerotic lesions similar.