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目的:观察蚁龙通痹汤对兔膝骨关节炎病理形态的影响,探讨其治疗骨关节炎可能的作用机制。方法:将40只日本雄性大耳白兔按体重分为4组,即正常组,模型组、蚁龙通痹汤组、葡立胶囊组,每组10只。除正常组外,其余各组均行右侧膝关节前交叉韧带切断建立骨关节炎病变模型。造模后第2天开始给药(葡立胶囊0.1 g·kg-1,蚁龙通痹汤生药0.6 g·kg-1,ig每日1次),连续4周。观察各组兔膝关节软骨及滑膜组织病理形态学改变,测定关节液中肿瘤坏死因子(TNF)-α和白介素(IL)-1β含量以及软骨下骨生物力学检测,并分析软骨中基质金属蛋白酶(MMP)-1、组织金属蛋白酶抑制因子(TIMP-1)的表达情况。结果:与模型组比较,蚁龙通痹汤组动物关节液IL-1β和TNF-α含量、软骨MMP-1和TIMP-1表达率、关节外在表现和软骨病理组织学变化,以及软骨最大载荷和弹性模量均出现明显差异改善。结论:蚁龙通痹汤能够减少兔膝骨关节炎关节液IL-1β和TNF-α的分泌,改善MMP-1和TIMP-1在软骨组织细胞中表达的平衡而达到对软骨组织保护的作用。
Objective: To observe the impact of ant dragon Tongbi decoction on pathological changes of rabbit knee osteoarthritis and to explore its possible mechanism of action in treatment of osteoarthritis. Methods: Forty male Japanese white rabbits were divided into 4 groups according to body weight: normal group, model group, ant dragon Tongbi decoction group and Puli capsule group, with 10 in each group. Except the normal group, all the other groups underwent right anterior cruciate ligament dissection to establish the model of osteoarthritis. On the second day after the model was established, the rats were administered with 0.1 g · kg-1 of Puli capsule, 0.6 g · kg-1 of antsongtongbi decoction, ig once a day for 4 weeks. The pathological changes of articular cartilage and synovial tissue in rabbits in each group were observed. The content of tumor necrosis factor (TNF) -alpha and interleukin-1β (IL-1β) in synovial fluid and the biomechanical test of subchondral bone were measured. The contents of matrix metal Protease (MMP) -1, tissue inhibitor of metalloproteinase (TIMP-1) expression. Results: Compared with the model group, the content of IL-1β and TNF-α in articular fluid, the expressions of MMP-1 and TIMP-1 in cartilage, the appearance of joint and the histopathological changes of cartilage in antongtongbi decoction group, Significant differences in load and modulus of elasticity improved. Conclusion: Ailongtongbi Decoction can reduce the secretion of IL-1β and TNF-α in the synovial fluid of rabbit knee osteoarthritis and improve the balance of MMP-1 and TIMP-1 expression in cartilage tissue to achieve the protection of cartilage tissue .