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目的:利用氟哌啶醇致僵直大鼠模拟帕金森病(PD)的运动不能,通过高频电刺激下丘脑后核(PH),观察大鼠僵直和运动能力的变化,从而探讨PH在PD治疗中潜在的应用价值。方法:将成年雄性SD大鼠随机分为PH刺激组、假刺激组和对照组,对PH刺激组和假刺激组大鼠双侧PH置入双极刺激电极,腹腔注射氟哌啶醇30 min后,PH刺激组给予持续高频电刺激(130 Hz,60μs,100μA),分别利用爬杆实验和跑步机实验评价大鼠僵直程度和运动能力。结果:腹腔注射氟哌啶醇1.0 mg/kg后,①大鼠呈僵直状态,其潜伏期为167.88±17.88 s,给予双侧PH高频电刺激后潜伏期显著缩短至77.5±21.27 s(P<0.01)。②跑步机试验显示大鼠跑动速度和跑动距离显著下降,分别为5.78±0.90 cm/s和8.06±4.35 m(P<0.01),给予双侧PH高频电刺激后显著提高跑动速度和跑动距离,分别为12.72±3.66 cm/s和98.61±96.75 m(P<0.01)。结论:腹腔注射氟哌啶醇可模拟帕金森病的僵直和运动不能症状,双侧高频电刺激PH可显著拮抗氟哌啶醇对大鼠僵直和运动不能的作用,提示PH为DBS治疗帕金森病运动不能的有效刺激靶点,为临床DBS刺激PH治疗PD提供实验依据。
OBJECTIVE: To investigate the effects of haloperidol on Parkinson’s disease (PD) in rats with simulated Parkinson’s disease (PD) by high-frequency electrical stimulation of the posterior nucleus of the hypothalamus (PH) and the changes of stiffness and motor capacity in rats. Potential therapeutic value. Methods: Adult male Sprague-Dawley rats were randomly divided into PH stimulation group, sham stimulation group and control group. Bipolar stimulation electrodes were implanted into bilateral PH of PH stimulation group and sham stimulation group, intraperitoneal injection of haloperidol 30 min The rats in PH stimulation group were given continuous high-frequency electrical stimulation (130 Hz, 60μs, 100μA). The stiffness and motor ability of rats were evaluated by climbing pole test and treadmill test respectively. RESULTS: After intraperitoneal injection of haloperidol 1.0 mg / kg, the rats were in a stiff state with an incubation period of 167.88 ± 17.88 s, and the incubation period was significantly shortened to 77.5 ± 21.27 s (P <0.01) after bilateral PH high-frequency electrical stimulation ). ② The treadmill test showed that the running speed and running distance of rats decreased significantly, which were 5.78 ± 0.90 cm / s and 8.06 ± 4.35 m (P <0.01), respectively. The bilateral PH high frequency electrical stimulation significantly increased the running speed And running distance were 12.72 ± 3.66 cm / s and 98.61 ± 96.75 m, respectively (P <0.01). Conclusion: Intraperitoneal injection of haloperidol can simulate the symptoms of Parkinson’s disease and motor dyskinesia. Bilateral high-frequency electrical stimulation of PH can significantly antagonize the effect of haloperidol on the stiffness and motor failure in rats, suggesting that PH is DBS treatment Jinssen disease can not effectively stimulate the target, in order to provide experimental evidence for clinical DBS stimulation PH treatment of PD.