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本研究目的是对柑桔种质资源组织培养保存技术的可能性进行评估。以幼龄(1～2年生)的凤梨甜橙(C.Sinensis(L)Osb.)为外植体的组织培养保存系统已被建立。包括二次培养:A、初培养:含芽的茎节段外植体,在附加有1mgBA/升,并含有BNS的培养基中培养(培养温度为26±1℃,相对湿度60j％,j光照时间每天16小时),生长出芽,转移到加有3mg NAA/升,并含有BNS的生根培养基中培养,生长出根,再转移到伸长培养基中培养,获得完整小植株。B、次培养:培养条件及培养基与初培
The purpose of this study was to evaluate the potential of tissue culture preservation techniques for citrus germplasm. Tissue culture preservation systems using young (1 to 2 years old) C. sinensis (L) Osb. As explants have been established. Including secondary culture: A. Primary culture: explants of buds containing buds were cultured in a medium supplemented with 1 mg BA / liter and containing BNS (culture temperature 26 ± 1 ° C., relative humidity 60 j%, j illumination time 16 hours a day), shoots were grown and transferred to rooting medium supplemented with 3 mg NAA / liter and containing BNS to grow roots and then transferred to an elongation medium for culturing to obtain intact plantlets. B, sub-culture: culture conditions and culture medium and initial training