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目前,对于机体感染甲型肝炎病毒(HAV)后能否产生干扰素有不同的看法。Lerin和Hahn等报道在HAV急性感染期,干扰素水平显著升高,而另一些作者则报道甲型肝炎患者在急性感染期和恢复期并未产生干扰素。本文作者对此进行了研究。经每毫升含10~2IU的人成纤维细胞干扰素(HuIFN-β)预处理的和未处理的两种二倍体人胚成纤维细胞,将其置于37℃16小时,去除上清液,用HAV接种这两种培养物,再置37℃孵育,并在一定间隔时间(0、2、
At present, there are different opinions on whether interferon can be produced after the body has invaded Hepatitis A virus (HAV). Lerin and Hahn et al. Reported a significant increase in interferon levels during acute HAV infection, while others reported that hepatitis A patients did not produce interferon during acute infection and convalescence. The author of this article has carried on the research. Two kinds of diploid human embryonic fibroblasts, pretreated and untreated with 10 ~ 2 IU human fibroblast interferon (HuIFN-β) per ml, were placed at 37 ° C for 16 hours, and the supernatant , Both cultures were inoculated with HAV, incubated at 37 ° C again and incubated at regular intervals (0, 2,