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目的检测类风湿关节炎(RA)急性期患者血清中瘦素(leptin)及相关因子水平的变化,探讨Leptin在RA急性期发生过程中可能存在的意义。方法用高灵敏的放射免疫分析法及酶联免疫吸附试验(ELISA)测定RA患者血清中Leptin、白细胞介素(IL)-1β、IL-2及C反应蛋白(CRP)的水平。结果①RA组男性[(3．84±0．50)ng/ml]与正常对照组中男性[(0．89±0．13)ng/ml]相比,血清Leptin水平显著升高(P＜0．05)、RA组女性[(9．77±0．52)ng/ml]与正常对照组中女性[(1．78±0．26)ng/ml]相比,血清Leptin水平显著升高(P＜0．05)；②RA组患者与正常对照组相比,血清IL-1β、IL-2及CRP水平均显著升高(P＜0．05)；③经相关性分析发现各组Leptin水平的变化与IL-1β、IL-2及CRP水平的变化均无显著相关性。结论提示Leptin可能作为一种独立的炎症应答因子,在RA急性期发生过程中发挥重要作用。
Objective To detect the changes of serum leptin and related factors in patients with acute rheumatoid arthritis (RA) and to explore the possible significance of leptin in the pathogenesis of RA. Methods Serum levels of Leptin, interleukin (IL) -1β, IL-2 and C-reactive protein (CRP) were measured by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA) Results ① The level of serum Leptin in RA group was significantly higher than that in male control group [(3.84 ± 0.50) ng / ml] [(0.89 ± 0.13) ng / ml] (P < 0.05). Serum Leptin level was significantly higher in women with RA (9.77 ± 0.52 ng / ml) than in women with normal controls (1.78 ± 0.26 ng / ml) (P <0.05). ②The levels of IL-1β, IL-2 and CRP in RA group were significantly higher than those in normal control group (P <0.05) .③According to the correlation analysis, The level of Leptin had no significant correlation with the changes of IL-1β, IL-2 and CRP levels. Conclusions Leptin may be an independent inflammatory response factor, which plays an important role in the occurrence of acute phase of RA.