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本文从分泌具有高中和活性和高保护作用的抗ＨＳＶ－１／ＨＳＶ－２型共同性糖蛋白Ｃ的鼠源性单克隆抗体（ＭｃＡｂ）的杂交瘤细胞１Ａ１２中提取胞浆总ＲＮＡ，以Ｏｌｉｇｏ（ｄＴ）１５为引物逆转录合成ｃＤＮＡ，用一对鼠抗体Ｋ链通用引物进行ＰＣＲ，扩增出１Ａ１２ＶＬ基因，并克隆入ｐＵＣ１８质粒中。序列分析结果表明，１Ａ１２ＶＬ基因由３３０ｂｐ组成，编码１１０个氨基酸，隶属小鼠轻链ｋ链４组。
In this study, total cytoplasmic RNA was extracted from 1A12, a murine monoclonal antibody (McAb) secreting anti-HSV-1 / HSV-2 common glycoprotein C with high and middle activity and high protective effect. Oligo (DT) 15 was reverse transcribed into cDNA. A pair of murine antibody K-chain universal primers were used for PCR. 1A12VL gene was amplified and cloned into pUC18 plasmid. Sequence analysis showed that the 1A12VL gene consisted of 330bp and encoded 110 amino acids, which belonged to 4 groups of mouse light chain kappa chain.