作者用分化剂佛波肉豆蔻酸酯(PMA)处理并以GM-CSF刺激人體性单核细胞系U937(来源于人组织淋巴瘤),纯化了IL-1受体拮抗蛋白(IRAP),其相对分子量(Mr)约为25K。在诱导步骤中,单用PMA处理所产生的IRAP水平很低,而采用GM-CSF刺激后产物水平至少增加十倍。上述纯化了的IRAP用N-葡聚糖酶消化(在变性条件下), The authors purified IL-1 receptor antagonist protein (IRAP) by treatment with the differentiation agent phorbol myristate (PMA) and stimulation of the human monocytic cell line U937 (derived from human tissue lymphoma) with GM-CSF The relative molecular weight (Mr) is about 25K. In the induction step, the level of IRAP produced by the PMA treatment alone was very low, whereas the product level was increased by at least ten-fold after stimulation with GM-CSF. The above purified IRAP was digested with N-glucanase (under denaturing conditions)