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目的探索小鼠早期砷暴露后,砷化物在肝、脑组织中的代谢与分布情况。方法采用氢化物发生-超低温捕集-原子吸收分光光度法测定母鼠和仔鼠肝和脑组织中无机砷(iAs)、一甲基胂(MMA)及二甲基胂(DMA)含量。结果各组母鼠肝组织中iAs、MMA和DMA含量及脑组织中iAs和DMA含量随饮水中砷浓度增加而增加;小鼠在生后15d肝组织中iAs含量增加,MMA含量在生后21 d增加;生后10、15、21 d,高砷组DMA含量分别为(0.020±0.005)、(0.031±0.012)、(0.239±0.076)μg/g,生后21 d DMA含量高于新生仔鼠和生后35 d仔鼠。高砷组脑组织iAs含量在仔鼠生后21 d达最高水平(0.088±0.042)μg/g;MMA在早期发育阶段未检测到;DMA平均水平在生后10d和15 d最低。结论乳房屏障可以有效阻止iAs和DMA进入母鼠乳汁中,成熟血脑屏障能够有效阻止iAs进入脑组织。
Objective To explore the metabolism and distribution of arsenic compounds in liver and brain after arsenic exposure in mice. Methods The contents of inorganic arsenic (iAs), methylarsenazo (MMA) and dimethylarsine (DMA) in the liver and brain tissues of maternal and offspring rats were determined by hydride generation-ultra-low temperature capture-atomic absorption spectrophotometry. Results The contents of iAs, MMA and DMA in the liver tissue and the content of iAs and DMA in the brain tissue increased with the increase of the arsenic concentration in the drinking water. The content of iAs in the liver of the mice increased 15 days after birth, d. The DMA contents in high arsenic group were (0.020 ± 0.005), (0.031 ± 0.012) and (0.239 ± 0.076) μg / g respectively at 10, 15 and 21 days after birth, Rats and pups 35 days after birth. The content of iAs in brain of high-arsenic group reached the highest level (0.088 ± 0.042) μg / g on the 21st day of life, while MMA was not detected in the early stage of development. The average level of DMA was the lowest on the 10th and 15th day after birth. Conclusion The breast barrier can effectively prevent the iAs and DMA from entering into the milk of maternal mice, and the mature blood-brain barrier can effectively prevent iAs from entering the brain.