【摘 要】
：
Objective To investigate the HIV quasispecies in patients with AIDS. Methods Blood samples were obtained from 5 patients with AIDS, the C2～C3 region of HIV- 1 en
【机 构】
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Shenzhen East Lake Hosp Zhenzhen 518020,
【出 处】
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China Medical Abstracts(Internal Medicine)
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Objective To investigate the HIV quasispecies in patients with AIDS. Methods Blood samples were obtained from 5 patients with AIDS, the C2～C3 region of HIV- 1 envelope gene (env) was amplified by nested polymerse chain reaction (PCR) from plasma of eacb patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous(ds) and nonsynonymous (dn) substitutions as well as ds/dn
Objective To investigate the HIV quasispecies in patients with AIDS. Methods Blood samples were obtained from 5 patients with AIDS, the C2 ~ C3 region of HIV-1 envelope gene (env) was amplified by nested polymerse chain reaction (PCR) from plasma of eacb patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous (ds) and nonsynonymous (dn) substitutions as well as ds / dn
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