论文部分内容阅读
目的检测并观察蛋白质精氨酸甲基转移酶2(protein arginine methyltransferase 2,PRMT2)及其新的剪接体PRMT2α、PRMT2β、PRMT2γ在雌激素受体α(estrogen receptorα,ERα)阳性和ERα阴性乳腺癌细胞株中的表达情况。方法提取ERα阳性乳腺癌细胞株(ZR-75-1、BT474、T47D、MCF-7)和ERα阴性的乳腺癌细胞株(MDA-MB-453、SK-BR-3、MDA-MB-231)总RNA并用RT-PCR方法检测PRMT2及其新剪接体mRNA的表达,采用内对照甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)同时进行相对定量以进行校正。结果不同乳腺癌细胞株中均可检测到PRMT2及其新剪接体的表达,在ERα阳性乳腺癌细胞株(ZR-75-1、BT474、T47D、MCF-7)中表达均较高,而在ERα阴性的乳腺癌细胞株(MDA-MB-453、SK-BR-3、MDA-MB-231)中表达均较低。结论 PRMT2与PRMT2α、PRMT2β、PRMT2γ基因在不同乳腺癌细胞株中存在差异表达,在ERα阴性乳腺癌细胞中低于ERα阳性乳腺癌细胞,PRMT2各剪接体有可能和PRMT2一样通过ERα信号途径影响乳腺癌的发生发展。
Objective To detect and observe the expression of protein arginine methyltransferase 2 (PRMT2) and its novel splice variants PRMT2α, PRMT2β and PRMT2γ in estrogen receptor α (ERα) positive and ERα negative breast cancer Cell lines in the expression. Methods The ERα positive breast cancer cell lines (ZR-75-1, BT474, T47D and MCF-7) and ERα negative breast cancer cell lines (MDA-MB-453, SK-BR-3 and MDA-MB-231) The total RNA was extracted and the expression of PRMT2 and its neo-splicing was detected by reverse transcription-polymerase chain reaction (RT-PCR). Meanwhile, relative quantification was performed by using internal control glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Results The expression of PRMT2 and its novel spliceosomes was detected in different breast cancer cell lines and was higher in ERα positive breast cancer cell lines (ZR-75-1, BT474, T47D and MCF-7) ERα negative breast cancer cell lines (MDA-MB-453, SK-BR-3, MDA-MB-231) were lower expression. Conclusions PRMT2 and PRMT2α, PRMT2β and PRMT2γ genes are differentially expressed in different breast cancer cell lines. In ERα negative breast cancer cells, the expression of PRMT2 is lower than that of ERα positive breast cancer cells. The PRMT2 spliceosomes may affect the mammary gland through the ERα signaling pathway like PRMT2 The occurrence and development of cancer.