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作为一种被广泛使用的氯代酰胺类除草剂,乙草胺进入环境中很难被降解。本研究从长期施用乙草胺的稻田土壤中分离筛选到一株以乙草胺为唯一碳源的菌株B-2,经形态、生理生化特性及16S r RNA基因序列同源性分析,最终鉴定为Klebsiella variicola。通过研究培养温度、初始p H值、菌体接种量、装液量、Na Cl浓度和乙草胺浓度对该菌株生长和降解的影响,确定了最佳生长条件。结果表明,菌株B-2以5%接种量接入浓度为100 mg/L乙草胺、p H 7.0的无机盐培养基(含0.1%Na Cl)中,30℃条件下培养5 d,对乙草胺的降解率可达90.31%。
As a widely used chloroamide herbicide, acetochlor is hardly degraded when it enters the environment. In this study, a strain B-2 with acetochlor as sole carbon source was isolated and screened from paddy soils with long-term application of acetochlor. The morphological, physiological and biochemical characteristics and 16S rRNA gene sequence homology analysis were used to identify the final strain For Klebsiella variicola. The optimum growth conditions were determined by studying the effects of temperature, initial pH, inoculum volume, liquid volume, NaCl concentration and acetochlor concentration on the growth and degradation of the strain. The results showed that strain B-2 was inoculated with 5% inoculum into 100 mg / L acetochlor and p H 7.0 inorganic salt medium containing 0.1% NaCl for 5 days at 30 ℃, Acetochlor degradation rate of up to 90.31%.